| Peer-Reviewed

In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India

Received: 18 April 2018     Accepted: 3 May 2018     Published: 30 May 2018
Views:       Downloads:
Abstract

The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.

Published in American Journal of Plant Biology (Volume 3, Issue 1)
DOI 10.11648/j.ajpb.20180301.13
Page(s) 12-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

In Vitro Regeneration, Musa Cultivar Gopi, Benzylaminopurine, Indole Butyric Acid, Clonal Propagation, Tripura

References
[1] INIBAP. International network for the improvement of banana and plantain. Annual Report,Montpellier, France.1998.
[2] FAO.On-line database of the Food and Agricultural Organization of the United Nations.http://apps.fao.org/default.html.2001.
[3] B. Aquil, T.J. Arif,N.B. Sarin,Q.M.R. Haq “Micro propagation and genetic transformation of banana for crop improvement and sustainable agriculture” J of Crop Sci, vol.3(2) pp. 64-77. 2012.
[4] L. Tripathi “Genetic engineering for improvement of Musa protection in Africal” Afr J Biotechnol, vol. 2(12), pp. 503-508. 2003.
[5] S.S. Cronauer, A.D. KriKorian “Multiplication of Musa from excised stem tips” Ann Bot (London),vol. 53, pp. 321-328. 1984.
[6] R. DoreSwamy, L. Sahijram“Micropropagation of banana from male floral apices culture in vitro”Sci Hortic, vol. 40(3) pp.181-188.1989.
[7] F.J. Novak, R. Afza, M. Van Duren, M. Perea-Dallos, B.V. Conger, T. Xiolang “Somatic embryogenesis and plant regeneration in suspension cultures of desert (AA, AAA) and cooking(AAB) bananas (Musa spp.)” Biotechnology, vol. 7, pp.154-159.1989.
[8] F.X. Cote, R. Domergue, S. Monmarson, J. Schwendiman, C. Teisson, J.V. Escalant“Embryogenic cell suspension from the male flower of Musa AAA cv. Grand nain”Physiol Plant, vol. 97, pp. 285-290.1996.
[9] M.A.K, Azad, M.N. Amin “Rapid clonal propagation of banana (Musa spp.) using in vitro culture of floral bud apex” Plant tissue cult, vol.1, pp. 1-9.2001.
[10] L. Hrahsel, A. Basu, L. Sahoo “In vitro Propagation and assessment of the genetic fidelity of Musa acuminate (AAA) cv. Vaibalhla Derived from Immature male Flowers” Appl Biochem Biotechnol,vol. 172, pp.1530-1539.2014.
[11] A. Rekha, S. C. Hiremath “Chromosome studies and Karyotype analysis of some triploid banana(Musa species) cultivars of AAA genomic group” J Hortl Sci, vol. 3(1), pp. 30-34.2008.
[12] A.K. Sharma, A. Sharma. “Chromosome techniques: theory and practice”. Butterworths.London.1965.
[13] N. Banerjee, A.K. Sharma “In vitro response as a reflection of genomic diversity in long-term cultures of Musa” Theoritical and Applied Genetics, vol. 76, pp. 733-736.1988.
[14] U. Habiba, S. Reja, M. L. Saha, M. R. Khan, S. Hadiuzzaman“Endogenous bacterial contamination during in vitro culture of table banana: Identification and prevention” Plant Tissue Cult,vol.12,pp.117-124.2002.
[15] S. Ahmed, A. Sharma, A. K. Singh, V. K. Wali, P. Kumari “In vitro multiplication of banana (Musasp.)cv. Grand Naine” Afr J Biotechnol, vol. 13(37), pp. 2696-2703.2014.
[16] P. Karule, V. Dalvi, A. Kadu, R. Chaudhari, V. R. SubramaniamA. B. Patil “A commercial micropropagation protocol for virupakshi (AAB) banana via apical meristem” Afr J Biotechnol, vol.15(11), pp. 401-407. 2016.
[17] K. Maharani, S. Beura, P.S. Munsi “A Fast Protocol for in vitro cloning of Banana (Musaacuminata) cv. Amritpani”Int J Curr Microbiol, App Sci, vol. 6(10), pp. 586-594.2017.
[18] R. L. Jarret “In vitro propagation and genetic conservation of bananas and plaintains” In IBPGR Advising committee in vitro storage, report of the third meeting (appendix), IBPGR, Rome, Italy.1986.
[19] D. Vuylsteke, Shoot tip culture for the propagation, conservation and exchange of Musa germ plasm.IBPGR. Rome. 1989.
[20] W.C. Wong “In vitro propagation of banana (Musa spp.): Initiation, proliferation and development of shoot tip cultivars on define media” Plant cell Tissue and Organ Cult,vol. 6, pp.159-166.1986.
[21] G. Arinative, P.R., Rubaihago, M. J. S. Magambo “Proliferation rate effects of cytokinins on banana(Musaspp) Cultivars”Sci Hortic, vol. 86, pp. 13-21.2000.
[22] M. Sheidai, H. Aminpoor, Z. Noormohammadi, F. Farahani “Genetic variation induced by tissue culture in Banana (Musa acuminate L.) cultivar Cavandish Dwarf Masoud” Gene Conserve, vol. 9, pp. 1-10. 2009.
[23] F. Farahani, H. Aminpoor, M. Sheidai, Z. Noormohammadi, M. H. Mazinan “An improved systemfor in vitro propagation of banana (Musa acuminate L.) cultivars” Asian J of Plant Sci, vol. 7, pp. 116-118. 2008.
[24] J.N. Buah, E. Danso, K. J. Taah, E. A. Abole, E.A. Bediako,J. Asieidu, R. Baidoo “The effects of different concentrations of cytokinins on the in vitro multiplication of plaintain (Musa sp.)” Biotech,vol. 9(3), pp. 343-347. 2010.
[25] N. Jafari, R.Y. Othman, N. Khalid “Effect of benzyl amino purine (BAP) pulsing on in vitro multiplication of Musa acuminata (banana)” Asian J of Plant Sci Res, vol. 10, pp. 2446-2454.2010.
[26] J. Lohidas, D. Sujin “Effect of growth hormones in the micropropagation of Banana cv. Matti” Plant Archives, vol.15 (1), pp. 307-314. 2015.
Cite This Article
  • APA Style

    Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. (2018). In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. American Journal of Plant Biology, 3(1), 12-16. https://doi.org/10.11648/j.ajpb.20180301.13

    Copy | Download

    ACS Style

    Rabindra Kumar Sinha; Puja Rani Saha; Anath Bandhu Das; Satya Narayan Jena; Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am. J. Plant Biol. 2018, 3(1), 12-16. doi: 10.11648/j.ajpb.20180301.13

    Copy | Download

    AMA Style

    Rabindra Kumar Sinha, Puja Rani Saha, Anath Bandhu Das, Satya Narayan Jena, Sangram Sinha. In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India. Am J Plant Biol. 2018;3(1):12-16. doi: 10.11648/j.ajpb.20180301.13

    Copy | Download

  • @article{10.11648/j.ajpb.20180301.13,
      author = {Rabindra Kumar Sinha and Puja Rani Saha and Anath Bandhu Das and Satya Narayan Jena and Sangram Sinha},
      title = {In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India},
      journal = {American Journal of Plant Biology},
      volume = {3},
      number = {1},
      pages = {12-16},
      doi = {10.11648/j.ajpb.20180301.13},
      url = {https://doi.org/10.11648/j.ajpb.20180301.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpb.20180301.13},
      abstract = {The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.},
     year = {2018}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - In Vitro Clonal Propagation of Musa Sp. Cultivar Gopi: A Palatable Banana of Tripura, India
    AU  - Rabindra Kumar Sinha
    AU  - Puja Rani Saha
    AU  - Anath Bandhu Das
    AU  - Satya Narayan Jena
    AU  - Sangram Sinha
    Y1  - 2018/05/30
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajpb.20180301.13
    DO  - 10.11648/j.ajpb.20180301.13
    T2  - American Journal of Plant Biology
    JF  - American Journal of Plant Biology
    JO  - American Journal of Plant Biology
    SP  - 12
    EP  - 16
    PB  - Science Publishing Group
    SN  - 2578-8337
    UR  - https://doi.org/10.11648/j.ajpb.20180301.13
    AB  - The present investigation has been concentrated on the establishment of an efficacious method for in vitro regeneration of a Musa cultivar Gopi of Tripura. Focus has been greatly anchored to the sterilization procedure for assuring successful aseptic culture establishment of this cultivar. The culture protocol for shoot induction and proliferation comprised of four different treatments in basal MS medium fortified with 6-Benzylaminopurine of varying concentrations. The optimum response was encountered in the treatment of BAP in MS medium at concentration of 8mg l-1 for shoot bud induction and multiplication. Subsequent shoot growth and multiplication was achieved through repeated subcultures in media containing lower concentration (4 mg l-1). Root initiation was stimulated and brought about by treatments with two different concentrations of Indole butyric acid. The best rooting was manifested in treatment with IBA at concentration of 2mg l-1. The present study is an approach towards successful establishment of a simple and rapid clonal propagation of a potent Musa cultivar Gopi having traditional and commercial value from this region of Tripura, North-east India.
    VL  - 3
    IS  - 1
    ER  - 

    Copy | Download

Author Information
  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

  • Department of Botany, Utkal University, Bhubaneswar, India

  • Genetics and Molecular Biology, Council of Scientific and Industrial Research-National Botanical Research Institute, Lucknow, India

  • Cytogenetics and Plant Biotechnology Lab., Department of Botany, Tripura University, Suryamaninagar, India

  • Sections