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Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels

Received: 23 May 2020     Accepted: 10 June 2020     Published: 28 June 2020
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Abstract

Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.

Published in American Journal of Nano Research and Applications (Volume 8, Issue 2)
DOI 10.11648/j.nano.20200802.13
Page(s) 28-34
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Silk Fibroin, Controlled Release, Hydrogels, Nanoparticles, Drug Delivery

References
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  • APA Style

    Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. (2020). Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. American Journal of Nano Research and Applications, 8(2), 28-34. https://doi.org/10.11648/j.nano.20200802.13

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    ACS Style

    Rezaul Haque Ansary; Tomal Roy; Ali Asraf; Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am. J. Nano Res. Appl. 2020, 8(2), 28-34. doi: 10.11648/j.nano.20200802.13

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    AMA Style

    Rezaul Haque Ansary, Tomal Roy, Ali Asraf, Sabina Easmin. Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels. Am J Nano Res Appl. 2020;8(2):28-34. doi: 10.11648/j.nano.20200802.13

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  • @article{10.11648/j.nano.20200802.13,
      author = {Rezaul Haque Ansary and Tomal Roy and Ali Asraf and Sabina Easmin},
      title = {Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels},
      journal = {American Journal of Nano Research and Applications},
      volume = {8},
      number = {2},
      pages = {28-34},
      doi = {10.11648/j.nano.20200802.13},
      url = {https://doi.org/10.11648/j.nano.20200802.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.nano.20200802.13},
      abstract = {Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - Preparation, Characterization and Antifungal Activity Studies of AgNPs Loaded Silk Fibroin Hydrogels
    AU  - Rezaul Haque Ansary
    AU  - Tomal Roy
    AU  - Ali Asraf
    AU  - Sabina Easmin
    Y1  - 2020/06/28
    PY  - 2020
    N1  - https://doi.org/10.11648/j.nano.20200802.13
    DO  - 10.11648/j.nano.20200802.13
    T2  - American Journal of Nano Research and Applications
    JF  - American Journal of Nano Research and Applications
    JO  - American Journal of Nano Research and Applications
    SP  - 28
    EP  - 34
    PB  - Science Publishing Group
    SN  - 2575-3738
    UR  - https://doi.org/10.11648/j.nano.20200802.13
    AB  - Silk fibroin hydrogels are promising materials for controlled drug delivery device due to their aqueous process ability, biocompatibility, and biodegradability. The research work is aimed to prepare silk fibroin (SF) hydrogels loaded with Ag nanoparticles and to evaluate its antifungal activities. The Silk fibroin hydrogels were formulated at 37°C using 2% (w/v) silk fibroin aqueous solution either by treating 50% (v/v) of ethanol, or 50% (v/v) of propanol, or 50% (v/v) of glycerol, respectively. Above these, the rate of gelation was sufficiently accelerated by addition of glycerol. The silk fibroin hydrogels and prepared silver nanoparticles (AgNPs) were characterized by using Scanning Electron Microscopy (SEM), Thermo Gravimetrical Analysis (TGA). The encapsulation efficiency and release profile of AgNPs were studied by UV-vis spectrometry. The particle size of AgNPs was measured by Malvern Zetasizer Nano and found 93±5 nm. The encapsulation efficiency and morphology of the hydrogels was affected by the formulation conditions. The in vitro release profile showed an initial burst release of AgNPs followed by controlled release for next 20 hours. The antifungal activity of AgNPs loaded SF hydrogels showed a positive response to Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be an effective biopolymeric matrix for antifungal applications.
    VL  - 8
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh

  • Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh

  • Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh

  • Department of Chemistry, Rajshahi University, Rajshahi, Bangladesh

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